武汉病毒所邓菲/沈姝团队通过宏基因组与血清筛查揭示巴基斯坦多地存在跨宿主传播新型蜱传病毒 | VS推荐

Fig. 1. Composition and abundance of viral communities in tick pools. The heatmap depicts the abundance profiles of different viral sequences across tick pools, expressed as log10-transformed Transcripts Per Million (TPM + 1) values. The data were log10-transformed and clustered based on a Euclidean distance matrix. Tick genera are indicated above the heatmap, viral families are shown on the left, and the corresponding viral species are listed on the right. . Viral species were taxonomically classified using BLASTx and are grouped according to family.

Fig. 2. Phylogenetic analysis of the identified tick-borne virus strains. Maximum-likelihood (ML) phylogenetic trees were constructed to determine the genetic relationships of the new strains of JMTV (A), LMTV (B), and BDTPV-2 (C and D) strains identified in this study using the full-length segment 1 (NS5-like protein) sequence, the RdRp sequence, and the L and S segments, respectively. All trees were inferred using the ML method with 10,000 ultrafast bootstrap replicates. The strains from this study are indicated by a red circle.

Supplementary Fig S1.Validation of recombinant antigen expression for LIPS assays.

Supplementary Fig S2. Serological responses to JMTV, LMTV, and BDTPV-2 in livestock (n=319), humans (n-253), and rats (n=214).

Supplementary Fig S3. Western blot analysis of JMTV antibody-positive sera. Purified viral particles were used as antigens.